Integrin Beta 1 Recombinant antibody, PBS Only

• 

  WB analysis using 82729-3-RR (same clone as 82729-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82729-3-RR (same clone as 82729-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82729-3-RR (same clone as 82729-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

• 

  IF Staining of Customer Sample using 82729-3-RR (same clone as 82729-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded Customer Sample customer using Integrin Beta 1 antibody (82729-3-RR, Clone: 1K20 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

Integrin Beta 1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 82729-3-PBS

Clone No.1K20

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ITGB1, Integrin beta-1, GPIIA, Glycoprotein IIa, FNRB

---

Product Information

82729-3-PBS targets Integrin Beta 1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Integrin Beta 1 fusion protein Ag3078
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 110-140 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• ENSEMBL Gene ID: ENSG00000150093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05556
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.