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Integrin Beta 1 Recombinant antibody

Integrin Beta 1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1K20

Cat no : 82729-3-RR

Synonyms

ITGB1, Integrin beta-1, GPIIA, Glycoprotein IIa, FNRB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin beta-1 Recombinant antibody (82729-3-RR)

    WB analysis using 82729-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin beta-1 Recombinant antibody (82729-3-RR)

    IHC staining of human placenta using 82729-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin beta-1 Recombinant antibody (82729-3-RR)

    IHC staining of human placenta using 82729-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, A431 cells, U-87 MG cells, HCT 116 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82729-3-RR targets Integrin Beta 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Integrin Beta 1 fusion protein Ag3078
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight110-140 kDa
GenBank Accession NumberBC020057
Gene Symbol Integrin beta 1
Gene ID (NCBI) 3688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 1 antibody 82729-3-RRDownload protocol
IHC protocol for Integrin Beta 1 antibody 82729-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols