Integrin Alpha V Recombinant antibody, PBS Only

• 

  WB analysis using 84883-5-RR (same clone as 84883-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 84883-5-RR (same clone as 84883-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (84883-5-RR, Clone: 242376D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84883-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84883-5-RR against Human Integrin Alpha V were performed. The affinity constant is 0.121 nM.

Integrin Alpha V Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84883-5-PBS

Clone No.242376D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ITGAV, 242376D3, CD51, Integrin alpha-V, Integrin alpha-V heavy chain

---

Product Information

84883-5-PBS targets Integrin Alpha V in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Integrin Alpha V fusion protein Ag25825
• Full Name: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Calculated Molecular Weight: 1048 aa, 116 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC136442
• Gene Symbol: Integrin alpha V
• Gene ID (NCBI): 3685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P06756
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha V, also known as CD51, is a type I transmembrane glycoprotein composed of an extracellular domain, a transmembrane region and cytoplasmic domain (PMID: 2430295; 1690718). It can form heterodimers with one of the five beta integrin subunits (beta 1, 3, 5, 6, 8) that recognize ligands containing the sequence Arg-Gly-Asp, such as vitronectin, fibronectin, and osteopontin (PMID: 37087799). Integrin alpha V mainly plays a role in extracellular matrix-mediated cell adhesion and migration, differentiation, wound healing, inflammation, tumorigenesis, proliferation, angiogenesis, and metastasis.