Tested Applications
Positive WB detected in | A549 cells, PC-3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66070 targets Integrin Alpha 3 in WB applications and shows reactivity with human, pig, mouse samples.
Tested Reactivity | human, pig, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Integrin Alpha 3 fusion protein Ag17293 Predict reactive species |
Full Name | integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) |
Calculated Molecular Weight | 1066 aa, 119 kDa |
Observed Molecular Weight | 120-140 kDa |
GenBank Accession Number | BC150190 |
Gene Symbol | Integrin alpha 3 |
Gene ID (NCBI) | 3675 |
RRID | AB_3084202 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P26006 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 488 Integrin Alpha 3 antibody CL488-66070 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |