CoraLite®594-conjugated Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H2C11

Cat no : CL594-67668

Synonyms

Ins 1, Ins1, Ins2 rs1, insulin I

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Validation Data Gallery

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  IF Staining of mouse pancreas using CL594-67668

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594-conjugated Ins1 antibody (CL594-67668, Clone: 1H2C11 ) at dilution of 1:300.

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Tested Applications

• Positive IF-P detected in: mouse pancreas tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67668 targets Ins1 in IF-P applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ins1 fusion protein Ag29988
• Full Name: insulin I
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: NM_008386
• Gene Symbol: Ins1
• Gene ID (NCBI): 16333
• RRID: AB_2920169
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.