CoraLite® Plus 488-conjugated Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

1H2C11

Cat no : CL488-67668

Synonyms

Ins 1, Ins1, Ins2 rs1, insulin I

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Validation Data Gallery

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  IF Staining of mouse pancreas using CL488-67668

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488-conjugated Ins1 antibody (CL488-67668, Clone: 1H2C11 ) at dilution of 1:300.

• 

  IF Staining of mouse pancreas using CL488-67668

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488-conjugated Ins1 antibody (CL488-67668, Clone: 1H2C11 ) at dilution of 1:300.

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Tested Applications

• Positive IF-P detected in: mouse pancreas tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-67668 targets Ins1 in IF-P applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ins1 fusion protein Ag29988
• Full Name: insulin I
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: NM_008386
• Gene Symbol: Ins1
• Gene ID (NCBI): 16333
• RRID: AB_2919515
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

Publications

Species	Application	Title
mouse	IF	iScience; Optimal HSF1 activation in response to acute cold stress in BAT requires nuclear TXNIP; Authors - Althea N Waldhart