IkB Alpha Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IkB Alpha Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6H7

Cat no : 66418-1-PBS

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

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Validation Data Gallery

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  WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66418-1-Ig (same clone as 66418-1-PBS)

  WB result of IkB Alpha antibody (66418-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66418-1-Ig (same clone as 66418-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66418-1-Ig (same clone as 66418-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66418-1-PBS targets IkB Alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IkB Alpha fusion protein Ag22040
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004983
• Gene Symbol: NFKBIA
• Gene ID (NCBI): 4792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.