IkB Alpha Monoclonal antibody

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• KD/KO Validated

IkB Alpha Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

29

CloneNo.

2G6H7

Cat no : 66418-1-Ig

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

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Validation Data Gallery

• 

  WB analysis using 66418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66418-1-Ig

  WB result of IkB Alpha antibody (66418-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Alpha transfected HEK-293 cells.

• 

  WB analysis of C6 using 66418-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66418-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells, HEK-293 cells, HSC-T6 cells, ROS1728 cells, Jurkat cells, Raji cells, K-562 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 28 publications below
• IF: See 1 publications below

Product Information

66418-1-Ig targets IkB Alpha in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IkB Alpha fusion protein Ag22040
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004983
• Gene Symbol: IkB Alpha
• Gene ID (NCBI): 4792
• RRID: AB_2881790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

Species	Application	Title
rat	WB	Acta Biomater; An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury.; Authors - Xu Liao
mouse	WB	Mol Ther Nucleic Acids; TAZ ameliorates the microglia-mediated inflammatory response via the Nrf2-ROS-NF-κB pathway.; Authors - Ji-Cheng Huang
human	WB	Bioengineered; Calcium-activated nucleotides 1 (CANT1)-driven nuclear factor-k-gene binding (NF-ĸB) signaling pathway facilitates the lung cancer progression; Authors - Fangfang Gao
mouse	WB	J Ethnopharmacol; Buyang Huanwu Decoction suppresses cardiac inflammation and fibrosis in mice after myocardial infarction through inhibition of the TLR4 signalling pathway; Authors - Guoyong Zhang
rat	WB	Foods; Gastroprotective Effects of the Aqueous Extract of Finger Citron Pickled Products against Ethanol-Induced Gastric Damage: In Vitro and In Vivo Studies; Authors - Xiaoai Chen
mouse	WB	Food Funct; DHA-enriched phosphatidylcholine from Clupea harengus roes regulates the gut-liver axis to ameliorate high-fat diet-induced non-alcoholic fatty liver disease; Authors - Li Qian