PE Rat IgG2b Isotype Control (LTF-2)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

LTF-2

Cat no : PE-65211

Synonyms

IgG2b, IgG2b Isotype Control, PE Rat IgG2b Isotype Control, Rat IgG2b Isotype Control

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Validation Data Gallery

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  FC experiment of BALB/c mouse splenocytes using PE-65211

  1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug PE Rat IgG2b Isotype Control (PE-65211, Clone: LTF-2) (blue) or 0.5 ug PE Anti-Mouse CD4 (PE-65104, Clone: GK1.5) (red). Cells were not fixed.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65211 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG2b Isotype Control
• RRID: AB_2883962
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The LTF-2 immunoglobulin is useful as an isotype-matched control. The LTF-2 immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.