Tested Applications
| Positive FC detected in |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65211-1-Ig targets IgG2b Isotype Control in IF, FC applications and shows reactivity with samples.
| Tested Reactivity | |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | IgG2b Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
The LTF-2 immunoglobulin is useful as an isotype-matched control. The LTF-2 immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG2b Isotype Control antibody 65211-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Rat IgG2b Isotype Control (LTF-2), catalog number 65211-1-Ig, has 3 published citations. These appear in Nature Cell Biology, Nature Communications, and Frontiers in Endocrinology. The associated research fields include coronary atherosclerosis progression, nonalcoholic steatohepatitis (NASH), and ovarian iron overload with aging signs in estrogen receptor 1-deficient mice.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Single-cell transcriptomics uncovers endothelial progenitor-like remodelling driving human coronary atherosclerosis progression. | ||
Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression. | ||
Front Endocrinol (Lausanne) Ovaries of estrogen receptor 1-deficient mice show iron overload and signs of aging |
