CoraLite® Plus 488 Armenian Hamster IgG Isotype Control (PIP)

IgG Isotype Control Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

PIP

Cat no : CL488-65210

Synonyms

CoraLite®488-conjugated Armenian Hamster IgG Isotype Control, IgG, PIP

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL488-65210

  1X10^6 BALB/c mouse splenocytes were surface stained with CoraLite® Plus 488-conjugated Armenian Hamster IgG Isotype Control (CL488-65210, Clone: PIP) (purple), or 0.5 ug FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) (green), or 0.5 ug FITC-Armenian Hamster IgG Isotype Control (black). Cells were not fixed.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65210 targets IgG Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG Isotype Control
• RRID: AB_2919273
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The PIP immunoglobulin is useful as an isotype-matched control. The PIP immunolglobulin is specific for Glutathione S-transferase and can be used as an isotype control for Armenian Hamster IgG antibodies. This antibody has been quality-tested for flow cytometry as negative control.