Armenian Hamster IgG Isotype Control (PIP)

IgG Isotype Control Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Applications

FC

Conjugate

Unconjugated

Publications

2

CloneNo.

PIP

Cat no : 65210-1-Ig

Synonyms

Armenian Hamster IgG Isotype Control, PIP, IgG

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 65210-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Armenian Hamster IgG Isotype Control (PIP) (65210-1-Ig, Clone: PIP) (red) or 0.5 ug Anti-Mouse CD3ε (145-2C11) (65060-1-Ig, Clone: 145-2C11) (green) and FITC anti-Armenian Hamster IgG, or unstained (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

65210-1-Ig targets IgG Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG Isotype Control
• RRID: AB_2918472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The PIP immunoglobulin is useful as an isotype-matched control. The PIP immunolglobulin is specific for Glutathione S-transferase and can be used as an isotype control for Armenian Hamster IgG antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Publications

Species	Application	Title
	FC	J Immunol; An Atlas of Grass Carp IgM+ B Cells in Homeostasis and Bacterial Infection Helps to Reveal the Unique Heterogeneity of B Cells in Early Vertebrates; Authors - Yi-Ru Pan
	FC	Aging Dis; Lymphocyte-Related Immunomodulatory Therapy with Siponimod (BAF-312) Improves Outcomes in Mice with Acute Intracerebral Hemorrhage; Authors - Zhiying Zhang