Anti-Human INSR/CD220 Rabbit Recombinant Antibody, PBS Only

INSR/CD220 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241687A10

Cat no : 98227-1-PBS

Synonyms

INSR, insulin receptor, HHF5, EC:2.7.10.1, CD220

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Validation Data Gallery

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  FC experiment of human PBMCs using 98227-1-RR (same clone as 98227-1-PBS)

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone: 241687A10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were not fixed. This data was developed using the same antibody clone with 98227-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98227-1-RR (same clone as 98227-1-PBS)

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone:241687A10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98227-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98227-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98227-1-RR against Human INSR/CD220 were performed. The affinity constant is 52.4 pM.

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Product Information

98227-1-PBS targets INSR/CD220 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: INSR
• GenBank Accession Number: BC117172
• Gene Symbol: INSR
• Gene ID (NCBI): 3643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).