INS Monoclonal antibody, PBS Only

INS Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B6A7

Cat no : 66198-1-PBS

Synonyms

ILPR, INS, IRDN

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Validation Data Gallery

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  IHC staining of human pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:5000 (under 10x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:5000 (under 40x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 66198-1-Ig (Insulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 66198-1-Ig (Insulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 66198-1-Ig (Insulin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The α cells of pancreas was labelled in green with 15954-1-AP (Glucagon antibody). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Somatostatin antibody (24496-1-AP, green). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 66198-1-Ig (Insulin antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66198-1-PBS targets INS in IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: INS fusion protein Ag8630
• Full Name: INS
• Calculated Molecular Weight: 110 aa, 12 kDa
• GenBank Accession Number: BC005255
• Gene Symbol: INS
• Gene ID (NCBI): 3630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.