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Somatostatin (1-116aa) Polyclonal antibody

Somatostatin (1-116aa) Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24496-1-AP

Synonyms

SMST, somatostatin, Somatostatin (1-116aa), SST, somatostatin

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Somatostatin (1-116aa) Polyclonal antibody (24496-1-AP)

    IHC staining of mouse pancreas using 24496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 24496-1-AP (Somatostatin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Somatostatin (1-116aa) Polyclonal antibody (24496-1-AP)

    IHC staining of mouse pancreas using 24496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 24496-1-AP (Somatostatin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Somatostatin (1-116aa) Polyclonal antibody (24496-1-AP)

    IF Staining of mouse pancreas using 24496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin antibody (24496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Somatostatin (1-116aa) Polyclonal antibody (24496-1-AP)

    IF Staining of mouse pancreas using 24496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin (1-116aa) antibody (24496-1-AP) at dilution of 1:2000 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L), CoraLite®647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Somatostatin (1-116aa) Polyclonal antibody (24496-1-AP)

    IF Staining of mouse pancreas using 24496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin (1-116aa) antibody (24496-1-AP) at dilution of 1:2000 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L), CoraLite®647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

Tested Applications

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24496-1-AP targets Somatostatin (1-116aa) in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Somatostatin (1-116aa) fusion protein Ag21294
Full Name somatostatin
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC032625
Gene Symbol SST
Gene ID (NCBI) 6750
RRIDAB_2918083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.

Protocols

Product Specific Protocols
IHC protocol for Somatostatin (1-116aa) antibody 24496-1-APDownload protocol
IF protocol for Somatostatin (1-116aa) antibody 24496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols