gp130/IL6ST Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00324-3

  Sandwich ELISA standard curve of MP00324-3, Human gp130 Recombinant Matched Antibody Pair - PBS only. 83284-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0024. 83284-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP00324-3

  Serum of sixteen individual healthy human donors was measured. The human gp130 concentration of detected samples was determined to be 123.3 ng/mL with a range of 38.7 - 330.5 ng/mL.

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  Sample test of MP00324-3

  Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of human gp130.

gp130/IL6ST {ptg:Brand} Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83284-4-PBS

Clone No.240992A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130

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Product Information

83284-4-PBS targets gp130/IL6ST as part of a matched antibody pair:

MP00324-3: 83284-7-PBS capture and 83284-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 6 signal transducer (gp130, oncostatin M receptor)
• Calculated Molecular Weight: 104 kDa
• GenBank Accession Number: NM_002184.3
• Gene Symbol: IL6ST
• Gene ID (NCBI): 3572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P40189-1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.