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gp130/IL6ST Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00324-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00324-3, Human gp130 Recombinant Matched Antibody Pair - PBS only. 83284-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0024. 83284-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

    Sandwich ELISA standard curve of MP00324-3

    Sandwich ELISA standard curve of MP00324-3, Human gp130 Recombinant Matched Antibody Pair - PBS only. 83284-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0024. 83284-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of human gp130.

    Sample test of MP00324-3

    Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of human gp130.

  • Serum of sixteen individual healthy human donors was measured. The human gp130 concentration of detected samples was determined to be 123.3 ng/mL with a range of 38.7 - 330.5 ng/mL.

    Sample test of MP00324-3

    Serum of sixteen individual healthy human donors was measured. The human gp130 concentration of detected samples was determined to be 123.3 ng/mL with a range of 38.7 - 330.5 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00324-3 targets gp130/IL6ST in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: gp130/IL6ST Recombinant antibody, PBS Only (Capture) 83284-7-PBS (240992G3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: gp130/IL6ST Recombinant antibody, PBS Only (Detector) 83284-4-PBS (240992A5). 100 μg. Concentration 1 mgl/ml.

Alternative gp130/IL6ST matched antibody pairs: MP00324-1, MP00324-2, MP01049-1, MP01049-2, MP01049-3, MP50086-1

Matched pair protein standard: Eg0654

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83284-7-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Synonyms IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130
GenBank accession numberNM_002184.3
Gene symbol IL6ST
UniprotP40189-1
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83284-4-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Synonyms IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130
GenBank accession numberNM_002184.3
Gene symbol IL6ST
UniprotP40189-1
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.