Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody, PBS Only

IL3RA/CD123 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241268E3

Cat no : 98107-1-PBS

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

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Validation Data Gallery

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  FC experiment of human PBMCs using 98107-1-RR (same clone as 98107-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (98107-1-RR, Clone: 241268E3) or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98107-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98107-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98107-1-RR against Human IL3RA/CD123 were performed. The affinity constant is 2.63 nM.

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Product Information

98107-1-PBS targets IL3RA/CD123 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: interleukin 3 receptor, alpha (low affinity)
• Calculated Molecular Weight: 378 aa, 43 kDa
• GenBank Accession Number: BC035407
• Gene Symbol: CD123
• Gene ID (NCBI): 3563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.