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CoraLite® Plus 647 Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

UCHL1

Cat no : CL647-65150

Synonyms

B220, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, UCHL1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150)

    FC experiment of human peripheral blood lymphocytes using CL647-65150

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD45RA (FITC-65108, Clone: HI100) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD45RO (CL647-65150, Clone: UCHL1). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150)

    FC experiment of human peripheral blood lymphocytes using CL647-65150

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD45RA (FITC-65108, Clone: HI100) and CoraLite® Plus 647-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65150 targets CD45RO in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen IL-2 dependent T cell line, CA1
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC014239
Gene Symbol CD45RO
Gene ID (NCBI) 5788
RRIDAB_2883714
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD45RO antibody CL647-65150Download protocol
Standard Protocols
Click here to view our Standard Protocols