IL-18 Monoclonal antibody

• Featured Product
• KD/KO Validated

IL-18 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

34

CloneNo.

5C6F8

Reviews (1)

Cat no : 60070-1-Ig

Synonyms

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

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Validation Data Gallery

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  WB analysis using 60070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60070-1-Ig

  WB result of Pre-IL18 antibody (60070-1-Ig, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells..

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  WB analysis using 60070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60070-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 60070-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug IL-18 Monoclonal antibody (60070-1-Ig, Clone:5C6F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, HepG2 cells, A549 cells
• Positive IHC detected in: human tonsillitis tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 28 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• ELISA: See 1 publications below

Product Information

60070-1-Ig targets IL-18 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-18 fusion protein Ag1063
• Full Name: IL-18
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC007461
• Gene Symbol: IL-18
• Gene ID (NCBI): 3606
• RRID: AB_2280158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Publications

Species	Application	Title
human	WB	Nature; Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome; Authors - Xuyan Shi
	WB	Stem Cells Transl Med; Mesenchymal Stem Cells-Derived Exosomes Alleviate Acute Lung Injury By Inhibiting Alveolar Macrophage Pyroptosis; Authors - Peipei Liu
	WB	J Neuroinflammation; GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome.; Authors - Zhangjin Qin
	WB	Int Immunopharmacol; Long non-coding RNA NEAT1 exacerbates NLRP3-mediated pyroptosis in allergic rhinitis through regulating the PTBP1/FOXP1 cascade; Authors - Yunliang Liu
rat	WB	J Ethnopharmacol; Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and Reperfusion--From the perspective of glial-vascular unit; Authors - Chang Zhou
human	IHC, WB	Int J Biol Sci; Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer.; Authors - Honglin Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bethany (Verified Customer) (01-05-2022)

Didn't work.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse kidney tissue