CoraLite®594-conjugated IL-18 Monoclonal antibody

• Featured Product
• KD/KO Validated

IL-18 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, hamster

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5C6F8

Cat no : CL594-60070

Synonyms

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, pre-IL18

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Validation Data Gallery

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  FC experiment of HeLa using CL594-60070

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human IL-18 (CL594-60070, Clone:5C6F8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60070 targets IL-18 in FC (Intra) applications and shows reactivity with human, hamster samples.

• Tested Reactivity: human, hamster
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-18 fusion protein Ag1063
• Full Name: IL-18
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC007461
• Gene Symbol: IL-18
• Gene ID (NCBI): 3606
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.