IL-18 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IL-18 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5C6F8

Cat no : 60070-1-PBS

Synonyms

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, pre-IL18

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Validation Data Gallery

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  WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

  WB result of Pre-IL18 antibody (60070-1-Ig, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells.. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60070-1-PBS targets IL-18 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-18 fusion protein Ag1063
• Full Name: IL-18
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC007461
• Gene Symbol: IL18
• Gene ID (NCBI): 3606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.