IL12RB1 Recombinant antibody

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  IF Staining of K-562 using 84391-4-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of THP-1 using 84391-4-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IL12RB1 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Cat No. 84391-4-RR

Clone No.241661D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1, CD212

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Tested Applications

• Positive IF/ICC detected in: K-562 cells, THP-1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84391-4-RR targets IL12RB1 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: interleukin 12 receptor, beta 1
• Calculated Molecular Weight: 662 aa, 73 kDa
• GenBank Accession Number: BC029121
• Gene Symbol: IL12RB1
• Gene ID (NCBI): 3594
• RRID: AB_3671926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42701
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.