IL12RB1 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01262-2

  Sandwich ELISA standard curve of MP01262-2, Human IL12RB1 Recombinant Matched Antibody Pair - PBS only. 84391-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00005. 84391-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01262-2

  The mean IL12RB1 concentration was determined to be 27.14 ng/mL in Jurkat cell extract based on a 1.9 mg/mL extract load and 48.02 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load.

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  IF Staining of THP-1 using 84391-4-RR (same clone as 84391-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84391-4-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 84391-4-RR (same clone as 84391-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84391-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01262-2

  Cytometric bead array standard curve of MP01262-2, IL12RB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84391-3-PBS. Detection antibody: 84391-4-PBS. Standard: RP00005. Range: 0.781-50 ng/mL.

IL12RB1 Uni-rAb^TM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84391-4-PBS

Clone No.241661D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1, CD212

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Product Information

84391-4-PBS targets IL12RB1 as part of a matched antibody pair:

MP01262-2: 84391-3-PBS capture and 84391-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: interleukin 12 receptor, beta 1
• Calculated Molecular Weight: 662 aa, 73 kDa
• GenBank Accession Number: BC029121
• Gene Symbol: IL12RB1
• Gene ID (NCBI): 3594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42701
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.