CoraLite® Plus 488 Anti-Rat IL-2 Rabbit Recombinant Antibody

IL-2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240264C7

Cat no : CL488-98046

Synonyms

Il2, interleukin 2, 240264C7

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Validation Data Gallery

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  FC experiment of rat splenocytes using CL488-98046

  1x10^6 untreated or PMA and ionomycin treated rat splenocytes were intracellularly stained with APC Anti-Rat CD4 and 0.25 ug CoraLite® Plus 488 Anti-Rat IL-2 Rabbit Recombinant Antibody (CL488-98046, Clone: 240264C7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin treated rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-98046 is IL-2 Fusion Protein expressed in E. coli.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 2
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_053836
• Gene Symbol: Il2
• Gene ID (NCBI): 116562
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).