Anti-Rat IL-2 Rabbit Recombinant Antibody

IL-2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Rat

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240264C7

Cat no : 98046-1-RR

Synonyms

Il2, interleukin 2, 240264C7

---

Validation Data Gallery

• 

  FC experiment of rat splenocytes using 98046-1-RR

  1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with APC Anti-Rat CD4 (OX-35) and 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of rat splenocytes using 98046-1-RR

  1x10^6 PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98046-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98046-1-RR against Rat IL2 were performed. The affinity constant is 0.36 nM.

---

Tested Applications

• Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98046-1-RR is IL-2 Fusion Protein expressed in E. coli.

• Tested Reactivity: Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 2
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_053836
• Gene Symbol: Il2
• Gene ID (NCBI): 116562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).