Anti-Rat IL-2 Rabbit Recombinant Antibody, PBS Only

IL-2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240264C7

Cat no : 98046-1-PBS

Synonyms

Il2, interleukin 2, 240264C7

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Validation Data Gallery

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  FC experiment of rat splenocytes using 98046-1-RR (same clone as 98046-1-PBS)

  1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with APC Anti-Rat CD4 (OX-35) and 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98046-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 98046-1-RR (same clone as 98046-1-PBS)

  1x10^6 PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98046-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98046-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98046-1-RR against Rat IL2 were performed. The affinity constant is 0.36 nM.

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Product Information

The immunogen of 98046-1-PBS is IL-2 Fusion Protein expressed in E. coli.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 2
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_053836
• Gene Symbol: Il2
• Gene ID (NCBI): 116562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.