CoraLite® Plus 488 Anti-Mouse IL-17A Rabbit Recombinant Antibody

IL-17A Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230038C7

Cat no : CL488-98005

Synonyms

Ctla 8, Ctla8, Il17, Il17a, interleukin 17A

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Validation Data Gallery

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  FC experiment of C57BL/6 Th17-polarized splenocytes using CL488-98005

  1x10^6 PMA and ionomycin treated Th17-polarized splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IL-17A (230038C7) Rabbit Recombinant Antibody (CL488-98005, Clone:230038C7), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5), and 0.25 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: PMA and ionomycin treated C57BL/6 Th17-polarized splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98005 targets IL-17A in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 17A
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: NM-010552
• Gene Symbol: Il17a
• Gene ID (NCBI): 16171
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.