Anti-Mouse IL-17A Rabbit Recombinant Antibody

IL-17A Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230038C7

Cat no : 98005-1-RR

Synonyms

Il17a, IL-17A, interleukin 17A, Interleukin17A, 230038C7, IL 17A, Il17

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Validation Data Gallery

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  FC experiment of C57BL/6 Th17-polarized splenocytes using 98005-1-RR

  1x10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Th17-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-17A (98005-1-RR, Clone: 230038C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:700, and 0.06 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98005-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98005-1-RR against Mouse IL-17A were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated Th17-polarized splenocytes
• Positive FC detected in: PMA and ionomycin stimulated Th17-polarized splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98005-1-RR targets IL-17A in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 17A
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: NM-010552
• Gene Symbol: Il17a
• Gene ID (NCBI): 16171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.