Anti-Human IL-10 (7B8G3), PBS Only

• 

  FC experiment of human PBMCs using 65286-1-Ig (same clone as 65286-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) or Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65286-1-Ig. This data was developed using the same antibody clone with 65286-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65286-1-Ig (same clone as 65286-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) (red) or Mouse IgG2b Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65286-1-Ig. This data was developed using the same antibody clone with 65286-1-PBS in a different storage buffer formulation.

IL-10 Monoclonal Antibody for FC

Cat No. 65286-1-PBS

Clone No.7B8G3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Synonyms

IL-10,IL10, 7B8G3, CSIF, IL 10, IL10

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65286-1-Ig

Product Information

65286-1-PBS targets IL-10 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-10 fusion protein HZ-1145
• Full Name: interleukin 10
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC104252
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.