CoraLite® Plus 488-conjugated IKZF1 Monoclonal antibody

IKZF1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D6B5

Cat no : CL488-66966

Synonyms

IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros

---

Validation Data Gallery

• 

  IF Staining of Jurkat using CL488-66966

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 IKZF1 antibody (CL488-66966, Clone: 1D6B5 ) at dilution of 1:200.

• 

  FC experiment of human PBMCs using CL488-66966

  1x10^6 human PBMCs were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated IKZF1 Monoclonal antibody (CL488-66966, Clone:1D6B5), and 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IF/ICC detected in: Jurkat cells
• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66966 targets IKZF1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig, rabbit samples.

• Tested Reactivity: human, mouse, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IKZF1 fusion protein Ag28637
• Full Name: IKAROS family zinc finger 1 (Ikaros)
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC018349
• Gene Symbol: IKZF1
• Gene ID (NCBI): 10320
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.