CoraLite®594-conjugated IgM Monoclonal antibody

human IgM Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D10B10

Cat no : CL594-66484

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, human IgM, IGHM, IgM, MU, VH

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-66484

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 human IgD antibody (CL647-16489, Magenta).

• 

  IF Staining of human tonsillitis using CL594-66484

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 human IgD antibody (CL647-16489, Magenta).

• 

  IF Staining of human appendicitis using CL594-66484

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-66484

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100.

• 

  IF Staining of human tonsillitis using CL594-66484

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue, human appendicitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66484 targets human IgM in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human IgM fusion protein Ag1459
• Full Name: immunoglobulin heavy constant mu
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC009851
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• RRID: AB_2883590
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.