HRP-conjugated human IgM Monoclonal antibody

human IgM Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

HRP

Publications

1

CloneNo.

2D10B10

Cat no : HRP-66484

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, human IgM, IGHM, MU, VH

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Validation Data Gallery

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  WB analysis using HRP-66484

  0.1 μL human plasma was subjected to SDS PAGE followed by western blot with HRP-66484 (HRP conjuaged mouse anti-human IgM) at dilution of 1:10000, 1:20000, 1:40000, incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

HRP-66484 targets human IgM in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human IgM fusion protein Ag1459
• Full Name: immunoglobulin heavy constant mu
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC009851
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• RRID: AB_2883841
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa). It's HRP conjugated.

Publications

Species	Application	Title
	WB	Mol Ther; Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer; Authors - Timothy J Smith