Human IgA Monoclonal antibody, PBS Only

Human IgA Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A5A5

Cat no : 60099-1-PBS

Synonyms

Human IgA Heavy Chain, IGHA1, 3A5A5, IGHA 2, IGHA 1

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Validation Data Gallery

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  WB analysis of human saliva using 60099-1-Ig (same clone as 60099-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 60099-1-Ig (same clone as 60099-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgA antibody (60099-1-Ig, Clone: 3A5A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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Product Information

60099-1-PBS targets Human IgA in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human IgA fusion protein Ag7417
• Full Name: immunoglobulin heavy constant alpha 1
• Calculated Molecular Weight: 496 aa, 53 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC016369
• Gene Symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).