CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody

IGF2BP3 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1L11

Cat no : CL750-81805

Synonyms

IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3

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Validation Data Gallery

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  WB analysis using CL750-81805

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL750-81805

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using CL750-81805

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody (CL750-81805, Clone:1L11)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HepG2 cells, K-562 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-81805 targets IGF2BP3 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IGF2BP3 fusion protein Ag6226
• Full Name: insulin-like growth factor 2 mRNA binding protein 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC065269
• Gene Symbol: IGF2BP3
• Gene ID (NCBI): 10643
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.