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IFT88 Recombinant antibody, PBS Only

IFT88 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230074E12

Cat no : 82886-2-PBS

Synonyms

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFT88 Recombinant antibody (82886-2-RR)

    WB analysis using 82886-2-RR (same clone as 82886-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82886-2-RR (IFT88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82886-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT88 Recombinant antibody (82886-2-RR)

    IF Staining of hTERT-RPE1 using 82886-2-RR (same clone as 82886-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT88 antibody (82886-2-RR, Clone: 230074E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82886-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using IFT88 Recombinant antibody (82886-2-RR)

    FC experiment of HEK-293 using 82886-2-RR (same clone as 82886-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug IFT88 Recombinant antibody (82886-2-RR, Clone:230074E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82886-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82886-2-RR

    Affinity and Kinetic Characterization of 82886-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82886-2-RR against Human IFT88 were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 82886-2-PBS is IFT88 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFT88 fusion protein Ag4980
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 88-95 kDa
GenBank Accession NumberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).