CoraLite® Plus 488-conjugated IFT81 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT81 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11744

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

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Validation Data Gallery

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  IF Staining of MDCK using CL488-11744

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:50, CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

• 

  IF Staining of MDCK using CL488-11744

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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  IF Staining of hTERT-RPE1 using CL488-11744

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells, MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11744 targets IFT81 in IF/ICC applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT81 fusion protein Ag2339
• Full Name: intraflagellar transport 81 homolog (Chlamydomonas)
• Calculated Molecular Weight: 676 aa, 80 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC029349
• Gene Symbol: IFT81
• Gene ID (NCBI): 28981
• RRID: AB_2919039
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.