IFT81 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IFT81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11744-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  WB analysis using 11744-1-AP (same clone as 11744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11744-1-AP (same clone as 11744-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11744-1-AP (same clone as 11744-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

  IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

  IP result of anti-IFT81 (IP:11744-1-AP, 4ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 5200ug. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11744-1-AP (same clone as 11744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

  IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent images of MDCK cells stained with IFT81 rabbit pAb (11744-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for IFT81, and Rhodamine-Goat anti-rabbit IgG for 66200-1-Ig. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

  IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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Product Information

11744-1-PBS targets IFT81 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT81 fusion protein Ag2339
• Full Name: intraflagellar transport 81 homolog (Chlamydomonas)
• Calculated Molecular Weight: 676 aa, 80 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC029349
• Gene Symbol: IFT81
• Gene ID (NCBI): 28981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.