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  • Featured Product
  • KD/KO Validated

IFT81 Polyclonal antibody

IFT81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11744-1-AP

Synonyms

CDV1R, CDV-1, CDV1, CDV 1R, CDV 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using IFT81 Polyclonal antibody (11744-1-AP)

    WB analysis of mouse testis using 11744-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IFT81 Polyclonal antibody (11744-1-AP)

    WB analysis using 11744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using IFT81 Polyclonal antibody (11744-1-AP)

    WB analysis of human brain using 11744-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IFT81 Polyclonal antibody (11744-1-AP)

    WB analysis of HEK-293 using 11744-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using IFT81 Polyclonal antibody (11744-1-AP)

    IP experiment of mouse brain using 11744-1-AP

    IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using IFT81 Polyclonal antibody (11744-1-AP)

    IP experiment of mouse brain using 11744-1-AP

    IP result of anti-IFT81 (IP:11744-1-AP, 4ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 5200ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IFT81 Polyclonal antibody (11744-1-AP)

    IHC staining of human prostate cancer using 11744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT81 Polyclonal antibody (11744-1-AP)

    IF Staining of hTERT-RPE1 cells using 11744-1-AP

    IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using IFT81 Polyclonal antibody (11744-1-AP)

    IF Staining of MDCK using 11744-1-AP

    Immunofluorescent images of MDCK cells stained with IFT81 rabbit pAb (11744-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) for IFT81, and Rhodamine-Goat anti-rabbit IgG for 66200-1-Ig.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT81 Polyclonal antibody (11744-1-AP)

    IF Staining of hTERT-RPE1 cells using 11744-1-AP

    IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT81 Polyclonal antibody (11744-1-AP)

    IF Staining of hTERT-RPE1 using 11744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using IFT81 Polyclonal antibody (11744-1-AP)

    IF Staining of MDCK using 11744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using IFT81 Polyclonal antibody (11744-1-AP)

    IF Staining of C2C12 using 11744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, HEK-293 cells, human brain tissue, rat testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, hTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11744-1-AP targets IFT81 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT81 fusion protein Ag2339
Full Name intraflagellar transport 81 homolog (Chlamydomonas)
Calculated Molecular Weight 676 aa, 80 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC029349
Gene Symbol IFT81
Gene ID (NCBI) 28981
RRIDAB_2121966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Protocols

Product Specific Protocols
WB protocol for IFT81 antibody 11744-1-APDownload protocol
IHC protocol for IFT81 antibody 11744-1-APDownload protocol
IF protocol for IFT81 antibody 11744-1-APDownload protocol
IP protocol for IFT81 antibody 11744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua
View Article
mouseIF

Nat Cell Biol

The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment.

Authors - Mu He
View Article
humanWB

Nat Cell Biol

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Authors - Malavika Raman
View Article
humanWB

Nat Commun

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Authors - Nicolas Taulet
View Article
mouseWB

Nat Commun

Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Authors - Kai Hao
View Article
mouseWB,IF

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
View Article