CoraLite® Plus 488-conjugated IFT140 Recombinant antibody

IFT140 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230231D5

Cat no : CL488-82947-7

Synonyms

---

Validation Data Gallery

• 

  IF Staining of hTERT-RPE1 using CL488-82947-7

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT140 antibody (CL488-82947-7, Clone: 230231D5 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

---

Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-82947-7 is IFT140 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IFT140 fusion protein Ag11119
• Full Name: intraflagellar transport 140 homolog (Chlamydomonas)
• Calculated Molecular Weight: 1462 aa, 165 kDa
• Observed Molecular Weight: 150-165 kDa
• GenBank Accession Number: BC035577
• Gene Symbol: IFT140
• Gene ID (NCBI): 9742
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).