Anti-Rat IFN-gamma Rabbit Recombinant Antibody, PBS Only

IFN-gamma Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

6K20

Cat no : 98001-1-PBS

Synonyms

IFN Gamma, Ifng, interferon gamma, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFNG2

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Validation Data Gallery

• 

  FC experiment of wistar rat splenocytes using 98001-1-RR (same clone as 98001-1-PBS)

  1X10^6 unstimulated or ConA, PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Wistar rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IFN-gamma (98001-1-RR, Clone: 6K20) and PE-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody. Cells were co-stained with 0.25 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98001-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 98001-1-PBS

  1x10^6 unstimulated or ConA, PMA and ionomycin stimulated (in the presence of protein transport inhibitors) rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IFN-gamma (98001-1-PBS, Clone: 6K20) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with 0.25 ug FITC Plus Anti-Rat CD4 (OX-35) (FITC-65179, Clone: OX-35). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98001-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98001-1-RR against Rat IFN-gamma were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC (Intra) detected in: ConA, PMA and ionomycin stimulated rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98001-1-PBS targets IFN-gamma in FC (Intra) applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interferon gamma
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_138880
• Gene Symbol: IFN-gamma
• Gene ID (NCBI): 25712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)