FITC Plus Anti-Rat CD4 (OX-35)
CD4 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Rat and More (1)
Applications
FC
Conjugate
FITC Plus Fluorescent Dye
CloneNo.
OX-35
Cat no : FITC-65179
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 1 publications below |
Product Information
FITC-65179 targets CD4 in FC applications and shows reactivity with Rat samples.
| Tested Reactivity | Rat |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MLR generated rat T helper lymphocytes |
| Full Name | Cd4 molecule |
| Gene Symbol | Cd4 |
| Gene ID (NCBI) | 24932 |
| RRID | AB_2883817 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Storage Buffer | PBS with 0.1% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD4 antibody FITC-65179 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis Lilrb4 ameliorates ileal injury in rats with hemorrhagic shock and suppresses the activation of NF-κB signaling pathway |
