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APC Anti-Rat CD4 (OX-35)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2a kappa

Reactivity

Rat

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

OX-35

Cat no : APC-65179

Synonyms

CD4, CD4 molecule, p55, W3/25

Validation Data Gallery

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  • Flow cytometry (FC) experiment of wistar rat splenocytes using APC Anti-Rat CD4 (OX-35) (APC-65179)

    FC experiment of wistar rat splenocytes using APC-65179

    1X10^6 Wistar rat splenocytes were surface stained with 0.25 ug APC Anti-Rat CD4 (APC-65179, Clone: OX-35) (blue) or 0.25 ug isotype control antibody (black). Cells were not fixed.

Tested Applications

Positive FC detected inrat splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65179 targets CD4 in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / Mouse IgG2a kappa
Class Monoclonal
Type Antibody
Immunogen MLR generated rat T helper lymphocytes
Full Name Cd4 molecule
Gene Symbol Cd4
Gene ID (NCBI) 24932
RRIDAB_2883043
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for APC CD4 antibody APC-65179Download protocol
Standard Protocols
Click here to view our Standard Protocols