IFITM3 Recombinant antibody

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  WB analysis using 81135-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81135-3-RR (IFITM3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 81135-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IFITM3 antibody (81135-3-RR, Clone: 242056C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of MCF-7 using 81135-3-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM3 Recombinant antibody (81135-3-RR, Clone:242056C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 81135-3-RR

  Biolayer interferometry (BLl) kinetic assays of 81135-3-RR against Human IFITM3 were performed. The affinity constant is 0.911 nM.

IFITM3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 81135-3-RR

Clone No.242056C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b, 242056C9

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, THP-1 cells
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81135-3-RR targets IFITM3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IFITM3 fusion protein Ag2285
• Full Name: interferon induced transmembrane protein 3 (1-8U)
• Calculated Molecular Weight: 133 aa, 15 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC006794
• Gene Symbol: IFITM3
• Gene ID (NCBI): 10410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01628
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression.