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IFITM1-Specific Monoclonal antibody
IFITM1-Specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 60074-1-Ig
Validation Data Gallery
|Positive WB detected in||TF-1 cells, K-562 cells, human testis tissue, HepG2 cells, HL-60 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||K-562 cells|
|Positive FC detected in||U2OS cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
60074-1-Ig targets IFITM1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||IFITM1-Specific fusion protein Ag2320|
|Full Name||interferon induced transmembrane protein 1 (9-27)|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14-17 kDa|
|GenBank accession number||BC000897|
|Gene ID (NCBI)||8519|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
|Product Specific Protocols|
|WB protocol for IFITM1-Specific antibody 60074-1-Ig||Download protocol|
|IHC protocol for IFITM1-Specific antibody 60074-1-Ig||Download protocol|
|IF protocol for IFITM1-Specific antibody 60074-1-Ig||Download protocol|
|IP protocol for IFITM1-Specific antibody 60074-1-Ig||Download protocol|
|FC protocol for IFITM1-Specific antibody 60074-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.
The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.
Cell Stem Cell
Sci Transl Med
Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.
Cell Host Microbe
Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Claudia (Verified Customer) (06-20-2022)
The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.