CoraLite®488-conjugated IFIH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFIH1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-21775

Synonyms

MDA5, Interferon-induced helicase C domain-containing protein 1, IFIH 1, Helicard, EC:3.6.4.13

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Validation Data Gallery

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  IF Staining of mouse skeletal muscle using CL488-21775

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-21775 (IFIH1 antibody) at dilution of 1:100.

• 

  IF Staining of mouse skeletal muscle using CL488-21775

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

• 

  IF Staining of mouse skeletal muscle using CL488-21775

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

• 

  IF Staining of mouse skeletal muscle using CL488-21775

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

• 

  IF Staining of mouse skeletal muscle using CL488-21775

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-21775 (IFIH1 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21775 targets IFIH1 in IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIH1 fusion protein Ag16467
• Full Name: interferon induced with helicase C domain 1
• Calculated Molecular Weight: 1025 aa, 117 kDa
• Observed Molecular Weight: 117-125 kDa
• GenBank Accession Number: BC111750
• Gene Symbol: IFIH1
• Gene ID (NCBI): 64135
• RRID: AB_2883087
• Conjugate: CoraLite®488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 491 nm / 516 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.