|Positive WB detected in||Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21775-1-AP is IFIH1/MDA5 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFIH1/MDA5 fusion protein Ag16467|
|Full Name||interferon induced with helicase C domain 1|
|Calculated molecular weight||1025 aa, 117 kDa|
|Observed molecular weight||117-125 kDa|
|GenBank accession number||BC111750|
|Gene ID (NCBI)||64135|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.
CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses.
BMC Vet Res
Curcumol inhibits encephalomyocarditis virus by promoting IFN-β secretion.
Endogenous Retrovirus Activation as a Key Mechanism of Anti-Tumor Immune Response in Radiotherapy.
J Gen Virol
Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.