IFIH1/MDA5 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFIH1/MDA5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

55

Cat no : 21775-1-AP

Synonyms

IFIH1, MDA5, IFIH 1, Helicard, EC:3.6.4.13

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Validation Data Gallery

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  WB analysis of Jurkat using 21775-1-AP

  WB result of IFIH1 antibody (21775-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFIH1 transfected Jurkat cells.

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  WB analysis of Jurkat using 21775-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1/MDA5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1/MDA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21775-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 21775-1-AP

  IP result of anti-IFIH1/MDA5 (IP:21775-1-AP, 4ug; Detection:21775-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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  IHC staining of human skeletal muscle using 21775-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21775-1-AP (IFIH1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 21775-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21775-1-AP (IFIH1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 21775-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using IFIH1/MDA5 antibody (21775-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab.

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Tested Applications

• Positive WB detected in: Jurkat cells, Raji cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 51 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 7 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

21775-1-AP targets IFIH1/MDA5 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIH1/MDA5 fusion protein Ag16467
• Full Name: interferon induced with helicase C domain 1
• Calculated Molecular Weight: 1025 aa, 117 kDa
• Observed Molecular Weight: 117-125 kDa
• GenBank Accession Number: BC111750
• Gene Symbol: IFIH1
• Gene ID (NCBI): 64135
• RRID: AB_10734593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
	WB,RIP	Cell Stem Cell; ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress.; Authors - Diana Guallar
human	WB	Nat Commun; MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.; Authors - Peipei Zhou
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio
human	WB	Nat Commun; HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction.; Authors - Hisashi Akiyama
human	WB	EMBO J; CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS; Authors - Jia Yi ; KD Validated