Anti-Human ICAM-1/CD54 Rabbit Recombinant Antibody

ICAM-1/CD54 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240646A11

Cat no : 98011-4-RR

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

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Validation Data Gallery

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  FC experiment of human PBMCs using 98011-4-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM-1 Rabbit Recombinant Antibody (98011-4-RR, Clone: 240646A11) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with anti-human CD19. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98011-4-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM-1 Rabbit Recombinant Antibody (98011-4-RR, Clone: 240646A11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98011-4-RR

  Biolayer interferometry (BLl) kinetic assays of 98011-4-RR against Human ICAM-1/CD54 were performed. The affinity constant is 2.15 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98011-4-RR is ICAM-1/CD54 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).