CoraLite® Plus 488-conjugated Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for WB, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

Cat no : CL488-10284

Synonyms

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Validation Data Gallery

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  WB analysis using CL488-10284

  Human plasma were subjected to SDS PAGE followed by western blot with CL488-10284 (Human IgG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of human tonsillitis using CL488-10284

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Human IgG antibody (CL488-10284) at dilution of 1:200.

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Tested Applications

• Positive WB detected in: human plasma
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-10284 targets Human IgG in WB, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Human IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50 kDa, 150 kDa
• RRID: AB_2918970
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.

Publications

Species	Application	Title
	IF	Oxid Med Cell Longev; Adropin Improves Radiation-Induced Myocardial Injury via VEGFR2/PI3K/Akt Pathway; Authors - Bingda Li