Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 10284-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of human plasma using 10284-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 10284-1-AP (Human IgG antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 10284-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG antibody (10284-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 5 publications below

Product Information

10284-1-AP targets Human IgG in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Human IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50 kDa
• RRID: AB_2877729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.

Publications

Species	Application	Title
	WB	Sci Adv; Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer.; Authors - Long Qin
		Int Immunopharmacol; LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.; Authors - Zhi-Min Wang
		Front Bioeng Biotechnol; Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.; Authors - Rui-Rui Guo
		Biosensors (Basel); Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber.; Authors - Yijian Huang
	CoIP	Int J Oral Sci; FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.; Authors - Xinyuan Zhao
	WB	Clin Exp Metastasis; Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer.; Authors - Yahui Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fatima (Verified Customer) (12-11-2018)

• Applications: Flow Cytometry,