Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG







Cat no : 10284-1-AP


Human IgG

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10284-1-AP targets Human IgG in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name Human IgG
Calculated molecular weight 155 kDa
Observed molecular weight 25 kda, 50 kDa, 150 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.


Product Specific Protocols
WB protocol for Human IgG antibody 10284-1-APDownload protocol
IHC protocol for Human IgG antibody 10284-1-APDownload protocol
IF protocol for Human IgG antibody 10284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Clin Exp Metastasis

Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer.

Authors - Yahui Huang

Front Bioeng Biotechnol

Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.

Authors - Rui-Rui Guo

Int Immunopharmacol

LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.

Authors - Zhi-Min Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Fatima (Verified Customer) (12-11-2018)

  • Applications: Flow Cytometry,